Details of experiments between O00151 and P35222
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PDLIM1
Protein A fragment: 1-87
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CTNNB1
Protein B fragment: 772-781
Protein B site: PBM
Protein B sequence: NQLAWFDTDL
Protein B construct: biotin-ttds-NQLAWFDTDL
Average holdup BI: 0.2
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.2
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.16