Details of experiments between the fragments K9N5R3~71-82 and Q96JH8~973-1075
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MERS-E
Protein A fragment: 71-82
Protein A site: PBM
Protein A sequence: KPPLPPDEWV
Protein A construct: biotin-DSKPPLPPDEWV
Protein B protein: RADIL
Protein B fragment: 973-1075
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.99
Experiment series 2
Protein A protein: MERS-E
Protein A fragment: 71-82
Protein A site: PBM
Protein A sequence: KPPLPPDEWV
Protein A construct: biotin-DSKPPLPPDEWV
Protein B protein: RADIL
Protein B fragment: 973-1075
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Normalized holdup BI: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.06