Details of experiments between K9N5R3 and O95049
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: MERS-E
Protein A fragment: 71-82
Protein A site: PBM
Protein A sequence: KPPLPPDEWV
Protein A construct: biotin-DSKPPLPPDEWV
Protein B protein: TJP3
Protein B fragment: 373-482
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.44
Holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.64
Experiment series 2
Protein A protein: MERS-E
Protein A fragment: 71-82
Protein A site: PBM
Protein A sequence: KPPLPPDEWV
Protein A construct: biotin-DSKPPLPPDEWV
Protein B protein: TJP3
Protein B fragment: 1-109
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.23
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.19
Experiment series 3
Protein A protein: MERS-E
Protein A fragment: 71-82
Protein A site: PBM
Protein A sequence: KPPLPPDEWV
Protein A construct: biotin-DSKPPLPPDEWV
Protein B protein: TJP3
Protein B fragment: 189-274
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.12
Holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: MERS-E
Protein A fragment: 71-82
Protein A site: PBM
Protein A sequence: KPPLPPDEWV
Protein A construct: biotin-DSKPPLPPDEWV
Protein B protein: TJP3
Protein B fragment: 373-482
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.52
Normalized holdup BI: 0.52
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.83
Experiment series 5
Protein A protein: MERS-E
Protein A fragment: 73-82
Protein A site: PBM
Protein A sequence: KPPLPPDEWV
Protein A construct: Biotin-ado-ado-KPPLPPDEWV
Protein B protein: TJP3
Protein B fragment: 373-482
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.51
Normalized holdup BI: 0.51
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.81