Details of experiments between A6NIM6 and Q8TF72
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SLC15A5
Protein A fragment: 570-579
Protein A site: PBM
Protein A sequence: SSIDLWETAL
Protein A construct: biotin-ttds-SSIDLWETAL
Protein B protein: SHROOM3
Protein B fragment: 21-113
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.81