Details of experiments between A6NIM6 and Q8TCX5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SLC15A5
Protein A fragment: 570-579
Protein A site: PBM
Protein A sequence: SSIDLWETAL
Protein A construct: biotin-ttds-SSIDLWETAL
Protein B protein: RHPN1
Protein B fragment: 504-602
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.35
Immobilized partner concentration in holdup experiment (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.41
Experiment series 2
Protein A protein: SLC15A5
Protein A fragment: 570-579
Protein A site: PBM
Protein A sequence: SSIDLWETAL
Protein A construct: biotin-ttds-SSIDLWETAL
Protein B protein: RHPN1
Protein B fragment: 504-602
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.48
Normalized holdup BI: 0.48
Immobilized partner concentration in holdup experiment (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5
Experiment series 3
Protein A protein: SLC15A5
Protein A fragment: 570-579
Protein A site: PBM
Protein A sequence: SSIDLWETAL
Protein A construct: biotin-ttds-SSIDLWETAL
Protein B protein: RHPN1
Protein B fragment: 504-602
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.56
Normalized holdup BI: 0.56
Immobilized partner concentration in holdup experiment (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 5.16
Experiment series 4
Protein A protein: SLC15A5
Protein A fragment: 570-579
Protein A site: PBM
Protein A sequence: SSIDLWETAL
Protein A construct: Biotin-ado-ado-SSIDLWETAL
Protein B protein: RHPN1
Protein B fragment: 504-602
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.47
Normalized holdup BI: 0.47
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.74