Details of experiments between the fragments A4U7F2~140-149 and P78352~155-249
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV67-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRPQRTQTQV
Protein A construct: biotin-ttds-SRPQRTQTQV
Protein B protein: DLG4
Protein B fragment: 155-249
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.46
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.01
Experiment series 2
Protein A protein: HPV67-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRPQRTQTQV
Protein A construct: biotin-ttds-SRPQRTQTQV
Protein B protein: DLG4
Protein B fragment: 155-249
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.49
Normalized holdup BI: 0.64
Immobilized partner concentration in holdup experiment (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.96