Details of experiments between A4U7F2 and Q92796
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: HPV67-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRPQRTQTQV
Protein A construct: biotin-ttds-SRPQRTQTQV
Protein B protein: DLG3
Protein B fragment: 221-314
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.35
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.79
Experiment series 2
Protein A protein: HPV67-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRPQRTQTQV
Protein A construct: biotin-ttds-SRPQRTQTQV
Protein B protein: DLG3
Protein B fragment: 124-219
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: HPV67-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRPQRTQTQV
Protein A construct: biotin-ttds-SRPQRTQTQV
Protein B protein: DLG3
Protein B fragment: 381-476
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: HPV67-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRPQRTQTQV
Protein A construct: biotin-ttds-SRPQRTQTQV
Protein B protein: DLG3
Protein B fragment: 221-314
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.55
Normalized holdup BI: 0.69
Immobilized partner concentration in holdup experiment (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 5.06
Experiment series 5
Protein A protein: HPV67-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRPQRTQTQV
Protein A construct: biotin-ttds-SRPQRTQTQV
Protein B protein: DLG3
Protein B fragment: 381-476
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.23
Normalized holdup BI: 0.29
Immobilized partner concentration in holdup experiment (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.3
Experiment series 6
Protein A protein: HPV67-E6
Protein A fragment: 140-149
Protein A site: PBM
Protein A sequence: SRPQRTQTQV
Protein A construct: biotin-ttds-SRPQRTQTQV
Protein B protein: DLG3
Protein B fragment: 124-219
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Normalized holdup BI: 0.14
Immobilized partner concentration in holdup experiment (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.89