Details of experiments between A4D2P6 and Q9Y2H0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GRID2IP
Protein A fragment: 272-370
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: DLGAP4
Protein B fragment: 983-992
Protein B site: PBM
Protein B sequence: IYVPEAQTRL
Protein B construct: biotin-ttds-IYVPEAQTRL
Average holdup BI: 0.16
Normalized holdup BI: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.08
Experiment series 2
Protein A protein: GRID2IP
Protein A fragment: 1-87
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: DLGAP4
Protein B fragment: 983-992
Protein B site: PBM
Protein B sequence: IYVPEAQTRL
Protein B construct: biotin-ttds-IYVPEAQTRL
Average holdup BI: -0.12
Normalized holdup BI: -0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.