Details of experiments between A4D2P6 and P03126
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: GRID2IP
Protein A fragment: 1-87
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B modification: (TPO)156
Protein B sequence: SSRTRRETQL
Protein B construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: GRID2IP
Protein A fragment: 1-87
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B sequence: SSRTRRETQL
Protein B construct: biotin-ttds-SSRTRRETQL
Average holdup BI: -0.02
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 7
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: GRID2IP
Protein A fragment: 272-370
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B sequence: SSRTRRETQL
Protein B construct: biotin-ttds-SSRTRRETQL
Average holdup BI: -0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: GRID2IP
Protein A fragment: 272-370
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B sequence: SSRTRRETQL
Protein B construct: biotin-ttds-SSRTRRETQL
Measured dissociation constant (10-6M): 69.71
Standard deviation of dissociation constant (10-6M): 49.65
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 4.16
Experiment series 5
Protein A protein: GRID2IP
Protein A fragment: 272-370
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B modification: (TPO)156
Protein B sequence: SSRTRRETQL
Protein B construct: Biotin-ttds-SSRTRREpTQL
Measured dissociation constant (10-6M): 0
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: GRID2IP
Protein A fragment: 1-87
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B modification: (TPO)156
Protein B sequence: SSRTRRETQL
Protein B construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: -0.06
Normalized holdup BI: -0.06
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 7
Protein A protein: GRID2IP
Protein A fragment: 272-370
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B modification: (TPO)156
Protein B sequence: SSRTRRETQL
Protein B construct: Biotin-ttds-SSRTRREpTQL
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: GRID2IP
Protein A fragment: 272-370
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B sequence: SSRTRRETQL
Protein B construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.1
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.11
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 3.86
Experiment series 9
Protein A protein: GRID2IP
Protein A fragment: 1-87
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B sequence: SSRTRRETQL
Protein B construct: biotin-ttds-SSRTRRETQL
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: GRID2IP
Protein A fragment: 1-87
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B sequence: SSRTRRETQL
Protein B construct: biotin-ttds-SSRTRRETQL
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: GRID2IP
Protein A fragment: 272-370
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B sequence: SSRTRRETQL
Protein B construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.08
Normalized holdup BI: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 3.74
Experiment series 12
Protein A protein: GRID2IP
Protein A fragment: 272-370
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B sequence: SSRTRRETQL
Protein B construct: Biotin-ado-ado-SSRTRRETQL
Average holdup BI: 0.25
Normalized holdup BI: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.3
Experiment series 13
Protein A protein: GRID2IP
Protein A fragment: 1-87
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B sequence: SSRTRRETQL
Protein B construct: Biotin-ado-ado-SSRTRRETQL
Average holdup BI: -0.08
Normalized holdup BI: -0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: GRID2IP
Protein A fragment: 272-370
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV16-E6
Protein B fragment: 149-158
Protein B site: PBM
Protein B sequence: SSRTRRETQL
Protein B construct: Biotin-ttds-SSRTRRETQL
Measured dissociation constant (10-6M): 0
Experiment method: SPR
PUBMED ID of publication: 30726710
Number of measurements: 1
The affinity was below the detection threshold of the assay.